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  1. 紀要類
  2. 横浜医学(1948-)
  3. 56巻(2005年)
  4. 2号

HER2を過剰発現する膵癌に対するHerceptin (trastuzumab), 5-FU (5-fluorouracil)併用療法の細胞傷害効果

https://ycu.repo.nii.ac.jp/records/905
https://ycu.repo.nii.ac.jp/records/905
18b8e65c-2d66-4006-9d94-5f32de7fe410
名前 / ファイル ライセンス アクション
KJ00004192672.pdf KJ00004192672.pdf (1.1 MB)
Item type [ELS]紀要論文 / Departmental Bulletin Paper(1)
公開日 2017-04-21
タイトル
タイトル HER2を過剰発現する膵癌に対するHerceptin (trastuzumab), 5-FU (5-fluorouracil)併用療法の細胞傷害効果
タイトル
言語 en
タイトル ENHANCED CYTOTOXIC EFFECT BY COMBINATION OF HERCEPTIN (TRASTUZUMAB) AND 5-FU (5-FLUOROURACIL) FOR HER2 OVEREXPRESSING PANCREATIC CANCER.
言語
言語 jpn
キーワード
主題Scheme Other
主題 ハーセプチン
キーワード
主題Scheme Other
主題 5-FU
キーワード
主題Scheme Other
主題 HER2
キーワード
主題Scheme Other
主題 膵癌
キーワード
主題Scheme Other
主題 化学療法
キーワード
言語 en
主題Scheme Other
主題 Herceptin
キーワード
言語 en
主題Scheme Other
主題 5-FU
キーワード
言語 en
主題Scheme Other
主題 HER2
キーワード
言語 en
主題Scheme Other
主題 pancreatic cancer
キーワード
言語 en
主題Scheme Other
主題 chemotherapy
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ departmental bulletin paper
雑誌書誌ID
収録物識別子タイプ NCID
収録物識別子 AN00246369
著者 佐伯, 博行

× 佐伯, 博行

WEKO 2175

佐伯, 博行

ja-Kana サエキ, ヒロユキ

Search repository
SAEKI, Hiroyuki

× SAEKI, Hiroyuki

WEKO 2176

en SAEKI, Hiroyuki

Search repository
著者所属(日)
値 秦野赤十字病院外科
著者所属(英)
言語 en
値 Department of Surgery, Hadano Red Cross Hospital
記事種別(日)
内容記述タイプ Other
内容記述 原著
記事種別(英)
内容記述タイプ Other
内容記述 ORIGINAL ARTICLE
抄録(日)
内容記述タイプ Other
内容記述 HER2(human epidermal growth factor receptor 2)は細胞膜貫通型チロシンキナーゼ受容体であり細胞の増殖,分化に関与している.膵癌の19.6~47.6%にHER2過剰発現が認められるがHerceptin(trastuzumab)の有効性や化学療法との併用効果に関しては不明である.今回,ヒト膵癌培養細胞株TRGを用いてHerceptinと5-FU(5-fluorouracil)の細胞傷害効果および併用効果をin vitroで検討した.TRG細胞はRT-PCR,Flow cytometryでHER2過剰発現が認められた.TRG細胞にHerceptinまたは5-FUを処理するとそれぞれ濃度依存性の細胞傷害効果が認められた.Herceptinと5-FUを併用処理するとそれぞれ単独処理よりも強い細胞傷害効果が認められた.Herceptinの抗体依存性細胞傷害活性(ADCC)を分析するためにTRG細胞にHerceptinまたは5-FUを処理した後,末梢血単核球(PBMC)を作用させた.PBMCの添加によりHerceptin,5-FUの細胞傷害効果は増強されたがHerceptinの方が増強効果が強く,ADCCの関与が示唆された.TRG細胞に5-FUを処理するとTRG細胞のHER2発現量が増加した.その結果Herceptinの細胞傷害効果が増強され5-FUとHerceptinの併用効果が得られたと考えられた.今回の研究でHerceptinと5-FUの併用療法はHER2を過剰発現する膵癌に対して有効な治療法となりうる可能性が示唆された.
抄録(英)
内容記述タイプ Other
内容記述 HER2, a transmembrane growth factor receptor, is overexpressed in 21 - 48% of pancreatic cancer. However, the efficacy of anti-HER2 antibody, Herceptin (trastuzumab), for pancreatic cancer patients has not been fully evaluated. In this study, the cytotoxic effect of Herceptin in combination with 5-FU (5-fluorouracil) for HER2 overexpressing human pancreatic cancer in vitro was investigated, using the human pancreatic adenocarcinoma cell line, TRG. The TRG cell line was positive for HER2 mRNA in RT-PCR analyses and overexpressed HER2 protein in flow cytomerty analyses. Either Herceptin or 5-FU concentration-dependently inhibited the growth of TRG cells. Combination of Herceptin and 5-FU resulted in significant inhibition of growth of TRG cells compared to either agent alone. We added peripheral blood mononuclear cells (PBMC) to TRG cells after treatment with Herceptin or 5-FU in order to analyze the effect of antibody-dependent cellular cytotoxicity (ADCC) by the chemotherapeutics. Incubation with PBMC enhanced the antiproliferative effect of Herceptin more than that of 5-FU, which could be the result of ADCC induced by Herceptin. This study showed that combination therapy with Herceptin and 5-FU could be effective for HER2 overexpressing pancreatic cancer patients.
書誌情報 横濱醫學
en : The Yokohama medical journal

巻 56, 号 2, p. 111-119, 発行日 2005-03-31
表示順
内容記述タイプ Other
内容記述 8
アクセション番号
内容記述タイプ Other
内容記述 KJ00004192672
ISSN
収録物識別子タイプ ISSN
収録物識別子 03727726
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